Journal: PLoS ONE

Article Title: Valinomycin Biosynthetic Gene Cluster in Streptomyces : Conservation, Ecology and Evolution

doi: 10.1371/journal.pone.0007194

Figure Lengend Snippet: Bacterial strains used in this study, including acronyms, origin and whether to produce VLM.

Article Snippet: S. GRIS1 (FIN) , Streptomyces griseus 1/k (DSM 41748) , Finland , + , DSMZ/ .

Techniques:

Journal: Nature Communications

Article Title: Inhibition of autophagy curtails visual loss in a model of autosomal dominant optic atrophy

doi: 10.1038/s41467-020-17821-1

Figure Lengend Snippet: a Representative z-projections of stacks of confocal images of phosphorylated-AMPK (p-AMPK, red) and GFP (blue) fluorescence in primary RGCs co-transfected as indicated and after 24 h immunostained for p-AMPK. The vector from the axonal hillock to the opposite side of the soma indicates the line used to measure p-AMPK fluorescence intensity in ( b ). Asterisks: axon. Bars, 20 μm. b Average ± SEM of p-AMPK fluorescence intensity along the vector drawn in the soma of RGCs co-transfected with GFP and the indicated plasmids in four independent experiments as in ( a ). EV, n = 73; Opa1, n = 69; Opa1 K301A , n = 75; Opa1 R905* , n = 73; Opa1 Q297V , n = 71 cells. c Average ± SEM ULK1 fluorescence intensity along the vector drawn in the soma of RGCs co-transfected with GFP and the indicated plasmids in three independent experiments as in ( a ) except that cells where immunostained for ULK1. EV, n = 21; Opa1 K301A , n = 26 cells. d Representative z-projections of stacks of confocal images of mtRFP (red) and YFP-LC3 (green, autophagosome-LC3, auto-LC3) in primary RGCs co-transfected as indicated. The cytoplasmic YFP-LC3 signal (cyto-LC3) is pseudocolored in grey for the sake of clarity. Boxed axonal regions and the soma region were magnified in the corresponding bottom panels. Bars, 20 μm. e , f Quantification of autophagosomes ( e ) and mitochondria ( f ) distribution in somas from three independent experiments as in ( d ). In ( e ), for EV: EV, n = 54; Opa1, n = 53; Opa1 K301A , n = 51; Opa1 R905* , n = 52; Opa1 Q297V , n = 52 cells; for AMPK DN : EV, n = 46; Opa1, n = 48; Opa1 K301A , n = 60; Opa1 R905* , n = 55; Opa1 Q297V , n = 51 cells. In ( f ) for EV: EV, n = 54; Opa1, n = 53; Opa1 K301A , n = 51; Opa1 R905* , n = 51; Opa1 Q297V , n = 52 cells; for AMPK DN : EV, n = 46; Opa1, n = 48; Opa1 K301A , n = 60; Opa1 R905* , n = 55; Opa1 Q297V , n = 51 cells. **** p < 0.0001 vs. EV in two-way ANOVA/Sidak’s test. g Quantification of mitochondrial content in RGCs axons from three independent experiments as in ( d ). For EV: EV, n = 53; Opa1, n = 46; Opa1 K301A , n = 51; Opa1 R905* , n = 47; Opa1 Q297V , n = 52 cells; for AMPK DN : EV, n = 59; Opa1, n = 53; Opa1 K301A , n = 59; Opa1 R905* , n = 50; Opa1 Q297V , n = 52 cells. **** p < 0.0001 vs. EV in two-way ANOVA/Sidak’s test. In box plots, centre line represents mean, bounds of boxes SEM, whiskers the 10th–90th percentiles; each dot represents independent experiments. Source data are provided as a Source Data file.

Article Snippet: The following antibodies were used: Atg7 (Sigma A2856, 1:500), Brn3a (Santa Cruz Biotechnology sc-31984, 1:500), GFP (Invitrogen A11120 and A11122, 1:1000), LC3B (Nanotools 0231, 1:500; Cell Signalling 2775 1:500), Phospho-AMPKα (Thr172) (Cell Signalling 2535, 1:200), β-Tubulin III (Sigma T8660, 1:1000), Ulk1 (Novus Biologicals, NBP2-41217, 1:200).

Techniques: Fluorescence, Transfection, Plasmid Preparation

Journal: Fermentation

Article Title: Effect of Substrate Characteristics on the Growth and Sporulation of Two Biocontrol Microorganisms during Solid State Cultivation

doi: 10.3390/fermentation6030069

Figure Lengend Snippet: Figure 1. Photos of (a) raw and (b) autoclaved parboiled rice, (c) Bacillus pumilus grown on parboiled rice at 50% moisture content, (d) raw and (e) autoclaved millet, (f) Streptomyces griseus grown on millet at 50% moisture content.

Article Snippet: B. pumilus (DSM 27) and S. griseus (DSM 40236) strains were purchased from Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures.

Techniques: